Comparing Antibody Titers after Vaccination in Dogs Using Elisa Reader with Image Processing Techniques

Abstract

Rabies is a zoonotic diseases that is caused by a virus of the genus Lyssa virus that attacks the central nervous system (CNS) and is transmitted through the bite of Rabies Transmitting Animals, especially dogs. Rabies can be prevented but is always fatal in humans if it infects the Central Nervous System (CNS). Vaccination has been used as one of the rabies prevention programs. A total of 83 samples were tested using Indirect ELISA (Enzyme-Linked Immunosorbent Assay) to identify antibody titers after rabies vaccination. Antibody titer correlated with absorbance value and concentration of standard solution. The absorbance value was read by ELISA reader and desktop scanner. The absorbance values were read at 450 nm and 620 nm as a reference using an ELISA reader and images from a desktop scanner were processed using the ImageJ application. The purpose of this study was to compare the obtained results using an ELISA reader as the gold standard and scanner images processed using ImageJ including sensitivity, specificity, and accuracy. There is no significant difference between the ELISA reader as the gold standard and alternative test equipment. The desktop scanner has a sensitivity of 97%, a specificity of 88.8% and an accuracy of 96%. The absorbance measurement method using an ELISA reader is expensive and difficult relatively to carry out in a laboratory with minimum financial support so that the image from desktop scanner processed using the ImageJ application is expected to be an alternative choice for an ELISA (Enzyme-Linked Immunosorbent Assay).